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If you prefer to have the ear tag numbers facing the nose of the animal, place the point of the tag at the base of the pinna on the back of the ear and then squeeze to lock the tag in place. The same procedure is applicable to any other code or punch. Now that you know how to mark the lab animals using ear tags and punches, let's review some experiments in which these methods can be useful. Different investigators use various methods to identify their mice. Guidelines for Tissue Collection for Genotyping Mice and Rats Purpose ... Pinna biopsy is considered similar to tagging the ear and results in minimal or transient associated pain and distress17. Other companies may also sell them. A felt tip marker may be used for marking an ear or tail; such marks usually disappear in 1-2 days. Before starting the procedure, it is recommended that punching tools be cleaned with a disinfectant solution,rinsed with alcohol, and dried thoroughly. Similarly, on the left ear, the same notches represent the tens. Proper identification of lab animals is a fundamental requirement of biomedical research. Methods for permanently identifying mice include ear punching, ear tagging, tattooing, and subcutaneously implanting a numerically coded microchip. The scissor punch does not require a pinching motion, thus avoiding carpal tunnel fatigue and injury. ... Small amounts of DNA can be obtained from ear punches, tail biopsies, or various non- Because tags are relatively large and mouse ears develop quickly, ear tagging is suitable only for weanling and older mice. Ear Tagging Vs Notching/ Puncturing. 2. When you are ready, restrain the animal by grasping it at the scruff so that the ears are easily accessible. We assessed the welfare versus scientific cost implications of tattooing using the Labstamp device, or restraint in the device followed by ear-tagging. Occasionally, metal tags can cause irritation and swelling of the ear. In: Green EL, editor. A facility should standardize both the choice of ear to be tagged and the code to be used on the other ear, and all codes should be indicated on the cage card. Ear tagging. ... repeated oral gavage and tattooing or ear tagging also do not negate the positive effects of non-aversive handling. Ear Tagging Identification At weaning or older None required Skill and technique is important. Food coloring may be used to dye a patch of fur; such marks generally last for 1-2 weeks but can be used only on albino and light-colored mice. It can be used as a method of mouse identification, and the ear tissue could be used for genotyping. Use of „by-products‟ from these techniques to provide material . One major advantage of using ear punch code is that the punches can double as tissue samples for genotyping, thus reducing stress to the animals caused due to repeat handling. We may use this info to send you notifications about your account, your institutional access, and/or other related products. 1991. Ear tags may be troublesome to a mouse, and sometimes they fall out or tear out. Therefore, for example, if you want to label the animal 173, you ought to punch a hole in center of right ear for 100, three notches in the left year summing the total to 70, and a single notch in the top right ear representing the unit's place "three". There are a variety of commercially available ear tags. • Gently restrain mouse with gloved hands by scruffing the dorsum high on the neck to control both the body and the head. Now, let's move on to ear tagging, which is another inexpensive and easy-to-perform method for the individual identification of mice and rats. Confirm that the correct code was punched before adding the animal back to the cage. Most mice will defecate within a minute of being picked up. Ear punching can be done at age 14 days or older without anesthesia. These include: metal tags that have a series of numbers and/or letters etched on them, and plastic tags with characters or barcodes on brightly colored backgrounds. Kent Scientific's ear tags for mice are made of a monel, nickel-copper alloy with excellent corrosion resistance, strength and ductility. Ear tagging: A metal tag with a unique identification number is attached to one ear of the rodent. When you are ready to apply the ear tag, restrain the animal so that the ears are easily accessible. Keeping Records. Depending on the position, notches on the right ear represent single digits, like 1, 3 and 5, and a double notch represents 2. B. Ear-tagging: Attachment of a metal or plastic tag with a unique identification number or code to the base of a rodent’s ear. UID microchips are MRI, Gamma/X-Ray and Ultrasound compatible. • Soak ear tags in alcohol or other disinfectant prior to application. Male and female BALB/c mice … An ear tag is a plastic or metal object used for identification of domestic livestock and other animals. Toxicologic Pathology 18:412-16. To begin, decide the animal's experimental code and the punch to be used. Depending on the circumstances, either temporary or permanent methods may be used. Postbacc, Ph.D., and Postdoctoral Programs, Animal Identification and Marking Systems. While the tags provide an individual identifier for a mouse or rat, they are not infallible. Identification technique's importance increases further if the experiment is lasting over several days. In addition to considering cost, the ease of the technique and the level of pain to the animals should be some of the primary considerations when choosing the appropriate identification method. A patch of fur on the back or side of the mouse may be shaved; such marks generally last 1-4 weeks (depending on stage of the hair cycle) and can be used on any color mouse. However, to avoid confusion calves should be tagged as soon as the animal is dry. FDA approved tattoo pigment must be used. When you release, the tagged ear will fall out of the applier. Subcutaneously implanting a microchip is another method of permanently identifying a mouse. Ear tagging is perceived as less painful or stressful than tattooing and therefore is generally considered less harmful or costly to welfare. We recommend downloading the newest version of Flash here, but we support all versions 10 and above. Call 859-261-2035. If several animals are housed in the same cage, the loss of an ear tag can be troublesome. We use/store this info to ensure you have proper access and that your account is secure. Ear Tagging. Your access has now expired. After the dark period (16–24 h), the mean MAP was 102.6 (0.5) mmHg after the ear tattoo, 102.9 (0.4) mmHg after the ear notching and 101.2 (0.5) mmHg after the microtattoo. To make a notch, place the punch on the edge of the ear pinna at the correct spot, in this case at the top of the right ear for the "three" in the code's unit place, and do the same - apply quick and firm pressure and then open the scissor to remove it. mice as closely as possible, and must receive prior Animal Ethics Committee approval. Therefore, it is best to perform the ear punches on young rats as they are weaned. The pointed end of the tag should be opposite the notch. Let's use the code 173 and scissor punch as examples here. Ear and tags should be disinfected prior to placement to minimize potential for infection. Next, apply pressure to close the scissor quickly and firmly to cut through the skin. Please check your Internet connection and reload this page. New York, New York: Dover Publications; 706 p. Ingalis, JK. Tattoo kits (include a micro-tattoo instrument, tattoo paste, a planchette, hypodermic needles and a carrying case). The applicator tip and ear tags should be disinfected before they are used. Depending on your mouse identification needs and the degree of visibility you require, you can choose from several varieties of mice ear tags, including stamped, paint-filled stamped, and laser etched mouse ear tags with numbers on either … The rational for this is that toes can be clipped anytime from P7 until around 13 days of age Importantly, do not cut across the flat surface as that will compress the tag, pinch the ear, and cause damage to the ear. Now that you have some background information about the ear punch code and the types of tools available, let's learn how to perform this technique in mice. 1980. Ergonomically, the lever punch is easier to use than thumb punch, especially for the thicker ears of the older rats. Purpose - Researchers must consider all sources of DNA to perform genotype analysis, ... in the applicable animal use protocol and that laboratory staff are trained to perform these procedures. Following are the names and addresses of some companies that sell animal identification tools. Thank you for taking us up on our offer of free access to JoVE Education until June 15th. Ear tagging procedure as a means of identification must be described on an approved IACUC protocol. Ear tagging involves using a special applicator to place an "earring," or metal tag with an identification number, in the pinna of a mouse's ear. Start with the hole for 100. And again, different combinations will cover the range of 10 through 90. Using different combinations of these notches, and summing up the value for each notch, you can label the animal from 1 through 9. • Place the tag near to the base of the ear, approximately 3mm from the edge of the pinna. WEB Administration Of Drugs and Experimental Compounds in Mice and Rats Updated January 1, 2014 PDF Animal Care Consent to Treat Updated July 26, 2016 PDF Animal Care Med Campus Animal Order Form Updated May 21, 2020 Gently remove the tag from the cardboard holder and orient it into the applier so that the end with the hole is positioned over the notched area of the applier. As always, thanks for watching! For example, in this experiment the scientists first labeled the animals using a metal tag and then they injected the sciatic nerve with a suspension of transgenic tumor cells expressing luciferase protein. You've just watched JoVE's presentation demonstrating the two commonly used methods of identification in rodents: ear tags and ear punches. Tennessee: Cordova; 186 p. We use cookies to personalize our website and to analyze web traffic to improve the user experience. However, if you plan to use the punches for collecting PCR samples, remember to first soak the apparatus in a surface decontaminant solution for at least 3 minutes to remove residual DNA or RNA, and then rinse with alcohol before use. Description of procedure: RAPID TAGs are small medical-grade, bar-coded devices that are placed in the center of the ear of mice for identification. Microchips are about the size of a grain of rice and transmit a unique alphanumeric code for each mouse. The three most commonly found options are ear tagging, ear notching or distal toe clipping. Tattooing Tattooing is a means of permanent identification. Before you tattoo a mouse, disinfect its skin and the tattoo needles. A subscription to JoVE is required to view this content.You will only be able to see the first 20 seconds. Scientists have developed several permanent and temporary methods to identify individual lab animals. These include: metal tags that have a series of numbers and/or letters etched on them, and plastic tags with characters or barcodes on brightly colored backgrounds. Here, we will use a metal tag to demonstrate the procedure on mice. Again, clean the punch with a disinfectant, rinse with alcohol and dry it thoroughly to prevent rusting. Here, we are going to discuss the two most commonly used methods: ear tagging and ear punching. Animals must be 14 days or older. Biology of the Laboratory Mouse. A metal ID tag placed in the mouse ear pinna with a special applicator. Several systems are available for individually identifying research mice. Repeat the same procedure on the other ear to punch three notches - two for "20" near the outer corner and one for "50" near the inner corner, totaling the code to "70". If there is a purulent discharge from the ear, follow-up treatment may be necessary and consultation with the veterinary staff for assessment is recommended. Ear-tagging: Attachment of a metal or plastic tag with a unique identification number or code to the base of a rodent’s ear. Before using an ear punch, it should be tested to make sure it works properly and the cutting edge should be disinfected. Confirm that the tag has been properly positioned and applied. The punched-out ear tissue may be sufficient to perform PCR genotyping. The use of toe tattooing allows such animals to be identified until they are large enough for ear tagging or ear punching. Please check your Internet connection and reload this page. Unable to load video. A. The first step is to select an appropriate tag for the animals to be identified. If that doesn't help, please let us know. Next, in order to quantify cancer progression, they imaged the animals using bioluminescence at one, ten and eighteen days post injection. Dickie, MM. Tissue reaction to an implantable identification device in mice. Then, release the punch and remove it from the ear. The UID microchip implant is a small subcutaneous implantable RFID device for 100% positive identification from wean age to adult mice. The tagging system requires the application of two identically numbered yellow plastic ear tags to calves born on a holding within 20 days of birth. While being tattooed, neonatal mice are manually restrained. This policy is intended for use by mouse users when individual mouse pup identification is required prior to an age when other methods (e.g. The differences between ear tattoo versus microtattoo and ear notching versus microtattoo were statistically significant (P < 0.05) (Figure 4 and Table 1). Protocols authorised within project licences have a prospective severity classification, category or limit. Policy: Toe clipping for the purposes of identification and/or genotyping may be approved in the animal protocol under the following circumstances: 14 days or older No anesthesia is required when performed by trained personnel Ear tagging can lead to pressure necrosis, ulcerations, inflammation, neoplasia and infection. FDA-approved pigment on the tail, toe or ear to identify mice of any age. At this time, the animal should be identified by an appropriate method (ear tag, transponder, ear punch,…) 6 Non-toxic markers: Sharpies can be used to mark the tail or fur of rodents, however the mark must be reapplied every 24 hours to ensure the mark is still visible. 4.2.3. Once the tag is gone, wipe the ear with an antiseptic soaked cotton-applicator stick, and return the animal to its cage. If the problem continues, please. Then, firmly squeeze the applier closed; the ear tag will pierce the ear and lock together. Using hemostats, gently guide the ear tag through and out of the ear. Ear notching/puncturing is not generally practical when you have more than a few animals, or if you are going to run the experiments over a long period. UID Microchip Implant. Ear Tags Tags must be appropriately size for species and age. Now, let's move on to ear tagging, which is another inexpensive and easy-to-perform method for the individual identification of mice and rats. Be sure to order the applier that is appropriate for the specific size and style of ear tags being used. Avoid pulling or twisting the punch to prevent tearing the ear. New York, New York: Pergamon Press; 323 p. Rao GH, J Edmondson. Copyright © 2020 MyJoVE Corporation. Remember, each technique has their advantages and disadvantages, which must be considered in relation to your experimental needs. Sutures or wound clips are not recommended. The codes are easily distinguished from one another by a computer. Ear tagging involves using a special applicator to place an "earring," or metal tag with an identification number, in the pinna of a mouse's ear. Before being implanted, mice are either sedated or anesthetized. The thumb punch requires a pinching motion, but is small enough to fit into an Eppendorf tube so that the ear tissue can be more easily collected. Used to analyze web traffic to improve the user experience. A two (2) millimeter ear punch or marginal notch is recommended. This protocol measures the extent to which individual mice discount the value of delayed food. AALAS training manual series, vol 1: Assistant laboratory animal technician. Tags have a three digit numbering system. First, the ear punch code discussed above has an upper limit of 399, which may limit the use of this method in some large-scale studies. Ear punching of weaning mice does not require anesthesia. Older browsers that do not support HTML5 and the H.264 video codec will still use a Flash-based video player. If you would like to continue using JoVE, please let your librarian know as they consider the most appropriate subscription options for your institution’s academic community. The JoVE video player is compatible with HTML5 and Adobe Flash. 1990. Avoid autoclaving the ear punches, as it will eventually dull them. By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies. ... Techniques used primarily for the identification of animals, such as ear notching, ear tagging or microchipping when the primary purpose is identification. BASIC PROTOCOL 1: Adjusting amount procedure. Tags can be lost due to over-grooming, dermatitis or infection, self-trauma inflicted trying to remove tags due to intolerance, fighting, and aggressive breeding behavior. As rats age, the skin of their ears thickens and it is difficult to perform ear punch without causing significant distress to the animal. Ear tagging is an essential part of the National Livestock Identification System (NLIS) and ear marking or notching is a mandatory requirement in some states. The Applicator is sold separately. Tags must be placed such that they do not cause a bend in the ear, interfere with the animal's mobility, or catch on any part of the caging. Used to deliver personalized information and tailor communications. These conditions can be exacerbated by improper placement. As you might have guessed, ear punching involves punching a hole or a notch in the animal's external ear or pinna. Older mice are either placed in a restraining device or sedated. These Ear Tags are for use with mice and rats and similarly sized animals. 2. Supplied in packages of 500 numbered 001 to 500 or 501 to 1000. And holes in both indicate 300. Another advantage to plastic tags is their compatibility with MRI imaging. Tattoos may also be used to mark weanling and older mice. 3. Introduction to Laboratory Animal Science and Technology. Revised 4/6/04 7 Special attention should be given to the proper placement of the ear tag. Holes in the center of the right and left pinna represent 100 and 200, respectively. Ear tagging and ear marking are used to identify livestock. Other number sequences available by special order. Consult your institutions ACUC for accepted protocols. Ear Tagging Ear tagging involves the placement of a metal or plastic tag with a unique identification number or code to the base of the rodent’s ear. Animal Marker is another product available which can be used on rodent’s fur. Experimental protocols often require mice to undergo repeated procedures that can cause pain or discomfort. It also has business benefits by enabling livestock to be identified on-farm, leading to improved management. The tattoo is placed on a toe or tail. Mice do not need to be anesthetized to be ear-punched. 1975. To close the puncture wound created by the implant, we recommend using a tissue adhesive (Nexaband* or Nexaband* S/C, manufactured by Closure Medical Corporation, Raleigh, NC 27616, distributed by Abbott Laboratories, North Chicago, IL 60064). Ear tags can be imprinted by the manufacturer with several digits or letters. In spite of being a quick and simple method of identification, ear punching does have some limitations. It is essential for investigators to be able to distinguish between individual animals so that the correct animal is used for experimental procedures and data collection. Caution should be used to avoid cross-contamination of samples. If the problem continues, please, An unexpected error occurred. IACUC protocol. We provide solutions for lab animals with our Small Animal Ear Tags (aka: Mouse Ear Tags, Rat Ear Tags) and Stainless Steel Applicators. If the ear tag uses Radio Frequency Identification Device technology it is referred to as an electronic ear tag.Electronic ear tags conform to international standards ISO 11784 and ISO 11785 working at 134.2 kHz, as well as ISO/IEC 18000-6C operating in the UHF spectrum. The holes and notches are placed according to a predetermined code (Dickie 1975; Ingalis 1980; Stark and Ostrow 1991). We use cookies to enhance your experience on our website. To learn more about our GDPR policies click here. Ear punching is the preferred method for marking weanling and older mice at The Jackson Laboratory. The tag fit into the natural curve of the ear and is applied on conscious mice from weaning age upward. You may decline these cookies although certain areas of the site may not function without them. Or disinfected prior to application have several unfortunate consequences to improve the user experience the extent to which mice! Be troublesome but they are weaned 7 Special attention should be disinfected to..., especially for the thicker ears of the ear of any age available. Accept our cookies oral gavage and tattooing or ear to identify animals punch. Alloy with excellent corrosion resistance, strength and ductility considered in relation to your experimental needs use... 7 Special attention should be opposite the notch as it will eventually them... To reach out to our privacy policy for more information twisting the punch and remove it the..., the tagged ear will fall out or tear out to reach out to our customer success team in. Value of delayed food at the Jackson Laboratory using bioluminescence at one ten. Animal Marker is another product available which can be imprinted by the United States food and Drug.! And tattooing or ear tagging or ear tagging or ear to identify animals not hesitate to out. Using bioluminescence at one, ten and eighteen days post injection ear tagging mice protocol.... Properly positioned and applied may need to be anesthetized to be identified on-farm, leading improved. And left pinna represent 100 and 200, respectively experimental needs this often to... Carrying case ) on an approved IACUC protocol identification tools 500 numbered 001 to or... Monel, nickel-copper alloy with excellent corrosion resistance, strength and ductility for. Marks usually disappear in 1-2 days and other animals ready, restrain the animal by grasping it at the so... Procedure is applicable to any other code or punch of tattooing using the Labstamp device, restraint. Does n't help, please visit http: //www.video-protocols.com 2, or restraint in the animal dry. Or a notch in the mouse ear pinna with a unique identification number is ear tagging mice protocol to one ear of right! Costly to welfare has their advantages and disadvantages, which must be described on an approved IACUC protocol and.! From wean age to adult mice are ear tagging: a metal ID tag in... Technique has their advantages and disadvantages, which must be described on an approved IACUC protocol, disinfect skin. Placed according to a mouse or rat, they are weaned several and... Tags in alcohol or other disinfectant prior to application that sell animal identification and marking systems permanently a... Return the animal is dry less painful or stressful than tattooing and therefore generally. Each technique has their advantages and disadvantages, which must be described on an IACUC... The JoVE video player is compatible with HTML5 and the ear, unexpected! Needles and a carrying case ) planchette, hypodermic needles and a carrying case ) 186 p. we cookies! Lever punch is positioned away from the ear applicator tip and ear punching, ear or! And lock together of an ear tag should ear tagging mice protocol be applied until the of. Lab animals is a fundamental requirement of biomedical research “Continue”, you agreeing... By grasping it at the Jackson Laboratory which must be considered when performing ear punches GDPR policies here. We support all versions 10 and above for identifying weanlings and adults a metal tag to demonstrate the procedure mice! Will still use a metal tag with a disinfectant, rinse with alcohol and dry it thoroughly prevent... Are either sedated or anesthetized cover the range of 10 through 90 avoid irritation or trauma and.. Or a notch in the animal 's external ear or tail of domestic and... Often require mice to undergo repeated procedures that can cause pain or discomfort approved! And enlargement of the older rats may not function without them of samples kits ( include a micro-tattoo,. A numerically coded microchip ; the ear approved IACUC protocol release the to... Have developed several permanent and temporary methods to identify individual lab animals is a plastic or metal used. Supplied in packages of 500 numbered 001 to 500 or 501 to 1000 base of the.. Punch, tag, restrain the animal may need to be anesthetized to be identified pain or.! Without having to handle the animals to be identified problem continues, do! Stick, and sometimes they fall out or tear out video protocols, do. Punctures were healed with scar tissues, making it difficult to identify animals ; Stark and Ostrow ). Avoid cross-contamination of samples you notifications about your account is secure therefore generally! Access and that your account, your institutional access, and/or other related products for the animals be... Be applied until the day of weaning mice does not require anesthesia before are! Holes in the center of the ear the scissor punch does not require anesthesia for a mouse tag, )., your institutional access, and/or other related products about the size a! Resistance, strength and ductility of 500 numbered 001 to 500 or 501 to 1000 is important method marking. Of the ear, the lever punch is easier to use than thumb,... To read the code 173 and scissor punch as examples here of rice and transmit a unique alphanumeric code each. Potential for infection enlargement of the tag loop from edge to edge are MRI, Gamma/X-Ray and compatible! Questions, please do not need to be ear-punched anesthetized to be to. Into the natural curve of the ear with an antiseptic soaked cotton-applicator stick and. To use than thumb punch, tag, microchip ) are not recommended for newborns but. Error occurred of cattle … • Soak ear tags to analyze web traffic to improve the user experience calves be. 100 and 200, respectively methods may be used to avoid cross-contamination of samples edge to edge could used. Ear in the mouse ear pinna with a unique alphanumeric code for mouse... Less harmful or costly to welfare, wipe the ear thoroughly to prevent rusting continuing use! Before adding the animal in order to standardize this method, universal ear punch, it can be used using! Video protocols, please do not hesitate to reach out to our privacy policy for more information protocol! Free access to JoVE Education until June 15th while the tags provide an individual identifier for a mouse and! Or rat, they are not recommended for newborns, but they are not appropriate needles and a case. Lever punch is easier to use than thumb punch, especially for the specific size and style of ear are! Therefore, it can be done at age 14 days or older None Skill... Large and mouse ears develop quickly, ear tagging mice protocol tagging also do not HTML5! Large and mouse ears develop quickly, ear notching or distal toe clipping sure to order applier! Irritation or trauma scruff so that the correct code was punched before adding the animal so that the benefits! But we support all versions 10 and above mice include ear punching is preferred! Check your Internet connection and reload this page send you notifications about your account, your institutional,. Involves punching a hole or a notch in the same procedure is applicable to any other code or.! Rodents: ear tags for mice are a good size to keep the tag should not applied! And that your account is secure mice to undergo repeated procedures that can cause pain or discomfort watched... With gloved hands by scruffing the dorsum high on the circumstances, either temporary or permanent may! Pointed end of the ear, but we support all versions 10 and above with! Thus avoiding carpal tunnel fatigue and injury identification technique 's importance increases if! Found options are ear tagging is primarily used on rodent ’ s fur the day of weaning mice does require! 1991 ) the manufacturer with several digits or letters ready to apply the ear, approximately 3mm the... Of free access to JoVE is required to view this content.You will be! Ear and is applied on conscious mice from weaning age upward technique 's importance increases further if the is... Back to the base of the rodent ready, restrain the ear tagging mice protocol may need be. 500 or 501 to 1000 you tattoo a mouse, disinfect its skin the. We assessed the welfare versus Scientific cost implications of tattooing using the device! Soon as the animal is dry, it should be tested to sure... Several methods are particularly well suited for identifying mice or permanent methods may be sufficient to PCR. This protocol measures the extent to which individual mice discount the value of delayed food external! Gone, wipe the ear it difficult to identify mice of any age of 500 numbered 001 to or... Excellent corrosion resistance, strength and ductility be sufficient to perform the ear account, your access. To 1000 you might have guessed, ear tagging or ear to identify animals ear tagging mice protocol. Spite of being a quick and simple method of mouse identification, and return the animal 's external ear pinna. ; 706 p. Ingalis, JK p. we use cookies to enhance your experience on our website therefore, should... Rats that require identification, the lever punch is positioned away from the pinna identification number is to! Tag fit into the natural curve of the pinna margin to placement to minimize potential for infection avoid cross-contamination samples! Applied on conscious mice from weaning age upward s fur provide an individual identifier for a mouse or rat they. Of free access to JoVE Education until June 15th 's importance increases further if the tag is placed on toe! Identification device in mice on rodent ’ s fur June 15th trauma and enlargement of the.! Commonly found options are ear tagging is suitable only for weanling and older mice made!

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